Title :
Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy
Author :
Wei, Shih-Chung ; Chuang, Tsung-Liang ; Lu, Hui-Hsin ; Chang, Chia-Chen ; Wang, Da-Shin ; Sung, Kung-Bin ; Lin, Chii-Wann
Author_Institution :
Institute of Biomedical Electronics and Bioinformatics, National Taiwan, University, Taiwan
fDate :
Aug. 30 2011-Sept. 3 2011
Abstract :
Tip-enhanced fluorescence of localized DNA replication by loop-mediated isothermal amplification (LAMP) is a potential way to observe real-time biological reaction confined in nanometer scale. We successfully coated Bst polymerase on the apex (∼100 nm) of an atomic force microscope (AFM) tip and performed localized LAMP reaction of hepatitis B virus (HBV). By using this tip-based reaction, the replicated HBV DNA can be directly imaged to be 400∼500 nm spots by using two-photon excitation fluorescence microscopy.
Keywords :
Biomedical optical imaging; DNA; Fluorescence; Isothermal processes; Microscopy; Photonics; Polymers; DNA Probes; DNA, Viral; Hepatitis B virus; Image Enhancement; Microscopy, Fluorescence, Multiphoton; Molecular Imaging; Molecular Probe Techniques; Nucleic Acid Amplification Techniques;
Conference_Titel :
Engineering in Medicine and Biology Society, EMBC, 2011 Annual International Conference of the IEEE
Conference_Location :
Boston, MA
Print_ISBN :
978-1-4244-4121-1
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2011.6090087
