Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy

Author

Wei, Shih-Chung ; Chuang, Tsung-Liang ; Lu, Hui-Hsin ; Chang, Chia-Chen ; Wang, Da-Shin ; Sung, Kung-Bin ; Lin, Chii-Wann

Author_Institution

Institute of Biomedical Electronics and Bioinformatics, National Taiwan, University, Taiwan

fYear

2011

fDate

Aug. 30 2011-Sept. 3 2011

Firstpage

490

Lastpage

493

Abstract

Tip-enhanced fluorescence of localized DNA replication by loop-mediated isothermal amplification (LAMP) is a potential way to observe real-time biological reaction confined in nanometer scale. We successfully coated Bst polymerase on the apex (∼100 nm) of an atomic force microscope (AFM) tip and performed localized LAMP reaction of hepatitis B virus (HBV). By using this tip-based reaction, the replicated HBV DNA can be directly imaged to be 400∼500 nm spots by using two-photon excitation fluorescence microscopy.

Keywords

Biomedical optical imaging; DNA; Fluorescence; Isothermal processes; Microscopy; Photonics; Polymers; DNA Probes; DNA, Viral; Hepatitis B virus; Image Enhancement; Microscopy, Fluorescence, Multiphoton; Molecular Imaging; Molecular Probe Techniques; Nucleic Acid Amplification Techniques;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, EMBC, 2011 Annual International Conference of the IEEE

Conference_Location

Boston, MA

ISSN

1557-170X

Print_ISBN

978-1-4244-4121-1

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2011.6090087

Filename

6090087