Title :
Alginate strings and their applications in spinal injury
Author :
Kanakasabai, Saravanan ; Wheatley, M.A.
Author_Institution :
Sch. of Biomed. Eng., Sci. & Health Syst., Drexel Univ., Philadelphia, PA, USA
Abstract :
We have optimized a method for producing strings of alginate bioconjugated with a laminin pentapeptide that aids in cell adhesion. These strings are 400-500 μm in thickness. In vitro studies have shown that rat (NB2a) and human (SHSY5Y) neuroblastoma cell lines adhere to and differentiate on these modified alginate strings. These strings are strong enough to be surgically transplanted onto the spinal cord, and may ultimately help in bridging the gap at the injury site
Keywords :
adhesion; biochemistry; cellular biophysics; surgery; alginate strings; carbodiimide chemistry; cell adhesion; cell anchorage; disrupted axons; in vitro studies; laminin pentapeptide bioconjugated; modified strings; neuroblastoma cell lines; neuronal attachment; neuronal differentiation; spinal injury applications; tissue engineering constructs; Adhesives; Antibiotics; Biological materials; Biomedical engineering; Bovine; Collision mitigation; Humans; Injuries; Needles; Nerve fibers;
Conference_Titel :
Bioengineering Conference, 2002. Proceedings of the IEEE 28th Annual Northeast
Conference_Location :
Philadelphia, PA
Print_ISBN :
0-7803-7419-3
DOI :
10.1109/NEBC.2002.999546
