Establishment of thromboangitis obliterans in rats

This project utilize surgery combine chemical method to establish rat model of thromboangitis obliterans. To appraise the model we use the method of pathology, immunology and molecular biology. The result shows that injection with laurate sodium to Femoral Artery can successfully establish rat model of thromboangitis obliterans that similar to human natural disease. Rat model pathological changes obviously, the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 mRNA rise significant in the disease region of rats, thromboxane B2 and anti-endothelial cell antibody increased significant in rats serum. These character was the same as thromboangitis obliterans in human. The rat model of thromboangitis obliterans can be used in further study, this offer a platform for the research of the pathologic mechanism and treatment of these disease.