Title :
Isolation of flower inducing gene SbCO in sorghum and construction of an inducible SbCO expression vector
Author :
Lv, Fangfang ; Xie, Xiaodong ; Ding, Bo ; Yang, Wenli ; Zhong, Silin ; Sun, Shoujun
Author_Institution :
TianJin Agric. Univ., Tianjin, China
Abstract :
We isolated the coding sequence a putative SbCO gene from Sorghum cultivar, Roma, by in silico sequence analysis and subsequently, reverse-transcription PCR. The DNA sequencing data revealed 92% similarity between SbCO and the Arabidopsis flowering time regulator CONSTANS. To investigate the biological function of SbCO in sorghum, we have constructed an inducible expression vector for SbCO based on Gateway cloning technology. This study lays a solid foundation for future analysis of flowering time control in Sorghum.
Keywords :
DNA; biochemistry; genetics; molecular biophysics; Arabidopsis flowering time regulator CONSTANS; DNA sequencing data; Sorghum cultivar; biological function; coding sequence; flower inducing gene SbCO; gateway cloning technology; inducible SbCO expression vector; putative SbCO gene; reverse-transcription PCR; Biological system modeling; Cloning; Encoding; Logic gates; Proteins; RNA; Expression vector; Gateway; SbCO gene; Sorghum;
Conference_Titel :
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location :
Nanjing
Print_ISBN :
978-1-4244-9172-8
DOI :
10.1109/RSETE.2011.5965953
