10C-2 High Frequency Ultrasound Characterization of Blood Clotting Process: Results Obtained With Plasma and Whole Blood

Author

Plag, C. ; Libgot, R. ; Gruel, Y. ; Patat, F. ; Ossant, F.

Author_Institution

Univ. Francois Rabelais, Tours

fYear

2007

fDate

28-31 Oct. 2007

Firstpage

884

Lastpage

887

Abstract

In recent studies (Libgot-Calle et al. , Ultras. Med. Biol. ,2007, in press) we showed that a simultaneous measurement of high frequency acoustic parameters could provide quantitative informations regarding the coagulation of human whole blood in vitro. HF acoustic parameters were measured both in double transmission (DT) and backscattering (BS) modes and were linked to biophysical and biochemical changes in clotting blood. The aim of this study was to discriminate the respective influence of red blood cells (RBC) and plasma proteins - especially fibrin network formation - on the evolution of our DT parameters and therefore on the mechanical properties of the clot. A 30 MHz ultrasonic transducer was used to estimate acoustic velocity and integrated attenuation coefficient (IAC) in whole blood, blood with various hematocrit, plasma, and plasma with added thrombin and the results underlined a major influence of RBC on our parameters.

Keywords

acoustic wave scattering; acoustic wave transmission; blood; haemorheology; ultrasonic applications; ultrasonic transducers; DT parameter evolution; HF acoustic parameters; acoustic backscattering; acoustic double transmission; acoustic velocity estimation; blood clot mechanical properties; blood clotting process; blood plasma proteins; fibrin network formation; frequency 30 MHz; high frequency ultrasound; human whole blood coagulation; integrated attenuation coefficient; red blood cells; ultrasonic transducer; Acoustic measurements; Blood; Coagulation; Frequency measurement; Humans; In vitro; Plasma measurements; Plasma properties; Ultrasonic imaging; Ultrasonic variables measurement;

fLanguage

English

Publisher

ieee

Conference_Titel

Ultrasonics Symposium, 2007. IEEE

Conference_Location

New York, NY

ISSN

1051-0117

Print_ISBN

978-1-4244-1384-3

Electronic_ISBN

1051-0117

Type

conf

DOI

10.1109/ULTSYM.2007.226

Filename

4409799