DocumentCode
2474596
Title
Purification of wheat esterase for detection of organophosphorus pesticide by a four-step extraction
Author
Bin Jiang ; Zhibiao Feng ; Liying Lu
Author_Institution
Dept. of Appl. Chem., NEAU, Harbin, China
fYear
2011
fDate
24-26 June 2011
Firstpage
7026
Lastpage
7029
Abstract
A new method for purification of wheat esterase was build which include four steps. The wheat esterase was purified to electrophoretically pure through ammonium sulfate saturation, ultrafiltration, Sephadex G150 and Procainamide affinity chromatography. The wheat esterase was puried 127 fold with specific activity 1264 U/mg after the four steps. The molecular weight of the wheat esterase estimated was 45kDa by SDS-PAGE.
Keywords
chromatography; purification; ultrafiltration; Procainamide affinity chromatography; Sephadex G150; ammonium sulfate saturation; four-step extraction; molecular weight; organophosphorus pesticide; ultrafiltration; wheat esterase purification; Biomedical optical imaging; Carbon; Optical films; Optical sensors; Physiology; Proteins; Purification; Procainamide affinity chromatography; Sephadex G150; purification; wheat esterase;
fLanguage
English
Publisher
ieee
Conference_Titel
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location
Nanjing
Print_ISBN
978-1-4244-9172-8
Type
conf
DOI
10.1109/RSETE.2011.5965982
Filename
5965982
Link To Document