Title :
Study on NPR1 gene transformation by ovary injection pathway into watermelon (citrullus vulgaris)
Author :
Li, Wenlan ; Sheng, Xiaowei ; Li, Jingjian ; Qin, Xinmin
Author_Institution :
Coll. of Chem. & Bioeng., Guilin Univ. of Technol., Guilin, China
Abstract :
Transformation of disease-resistant gene NPR1 to Watermelon was studied by ovary injection pathway after artificial pollination . Transgenic plants were obtained. Studies showed the injection of DNA solution at about 24 hours after pollination resulted in the highest transformation rate. PCR and southern blotting hybridization results showed that NPR1 gene were transformed into Watermelon.
Keywords :
biological techniques; botany; cellular biophysics; genetics; molecular biophysics; Citrullus vulgaris; DNA solution injection; NPR1 gene transformation; PCR; artificial pollination; disease resistant gene transformation; ovary injection pathway; southern blotting hybridization; transgenic plants; watermelon; Cloning; DNA; Diseases; Electron tubes; Immune system; Manuals; Presses; NPR1 gene; Ovary injection pathway; Transformation; Watermelon;
Conference_Titel :
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location :
Nanjing
Print_ISBN :
978-1-4244-9172-8
DOI :
10.1109/RSETE.2011.5966010
