• DocumentCode
    2475896
  • Title

    LQG-based tracking of multiple fluorescent particles in two-dimensions in a confocal microscope

  • Author

    Shen, Zhaolong ; Andersson, Sean B.

  • Author_Institution
    Dept. of Mech. Eng., Boston Univ., Boston, MA, USA
  • fYear
    2009
  • fDate
    10-12 June 2009
  • Firstpage
    1682
  • Lastpage
    1687
  • Abstract
    This paper describes a scheme to track multiple fluorescent particles diffusing in two dimensions. Fluorescence intensities are measured using a scanning-stage confocal setup. We develop a combined model for the position of the particles and the dynamics of the piezo-stage and design a linear-quadratic-Gaussian controller to drive the overall estimation error to zero. The scheme is illustrated through simulation.
  • Keywords
    fluorescence; linear quadratic Gaussian control; optical microscopes; particle tracks; LQG-based tracking; confocal microscope; error estimation; linear-quadratic-Gaussian controller; multiple fluorescent particle tracking; Actuators; Biological system modeling; Charge coupled devices; Charge-coupled image sensors; Estimation error; Fluorescence; Image processing; Microscopy; Particle measurements; Particle tracking;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    American Control Conference, 2009. ACC '09.
  • Conference_Location
    St. Louis, MO
  • ISSN
    0743-1619
  • Print_ISBN
    978-1-4244-4523-3
  • Electronic_ISBN
    0743-1619
  • Type

    conf

  • DOI
    10.1109/ACC.2009.5160593
  • Filename
    5160593