Title :
LQG-based tracking of multiple fluorescent particles in two-dimensions in a confocal microscope
Author :
Shen, Zhaolong ; Andersson, Sean B.
Author_Institution :
Dept. of Mech. Eng., Boston Univ., Boston, MA, USA
Abstract :
This paper describes a scheme to track multiple fluorescent particles diffusing in two dimensions. Fluorescence intensities are measured using a scanning-stage confocal setup. We develop a combined model for the position of the particles and the dynamics of the piezo-stage and design a linear-quadratic-Gaussian controller to drive the overall estimation error to zero. The scheme is illustrated through simulation.
Keywords :
fluorescence; linear quadratic Gaussian control; optical microscopes; particle tracks; LQG-based tracking; confocal microscope; error estimation; linear-quadratic-Gaussian controller; multiple fluorescent particle tracking; Actuators; Biological system modeling; Charge coupled devices; Charge-coupled image sensors; Estimation error; Fluorescence; Image processing; Microscopy; Particle measurements; Particle tracking;
Conference_Titel :
American Control Conference, 2009. ACC '09.
Conference_Location :
St. Louis, MO
Print_ISBN :
978-1-4244-4523-3
Electronic_ISBN :
0743-1619
DOI :
10.1109/ACC.2009.5160593
