Dual-axes confocal microscopy for clinical skin imaging

Author

Piyawattanametha, W. ; Ra, H. ; Mandella, M.J. ; Gonzalez, E. ; Kaspar, R. ; Kino, G.S. ; Contag, C.H. ; Solgaard, O.

Author_Institution

Edward L. Ginzton Lab., Stanford Univ., Stanford, CA, USA

fYear

2009

fDate

17-20 Aug. 2009

Firstpage

3

Lastpage

4

Abstract

We demonstrate MEMS based near infrared fluorescence dual-axes confocal (DAC) imaging in a 10-mm-diameter microscope for three-dimensional (3-D) imaging of in vivo human skin. The probe is used to reveal mosaicing two-dimensional (2-D) en face sub-surface histology at depths of up to 250 mum. The probe has a maximum field of view (FOV) of 800times450 mum² and a maximum acquisition rate of 10 frames/second. The transverse and axial resolutions are 5 mum and 7 mum, respectively.

Keywords

biomedical optical imaging; micromechanical devices; optical microscopy; probes; skin; MEMS; clinical human skin imaging; depth 250 mum; dual-axes confocal microscopy; near-infrared fluorescence dual-axes confocal imaging; probe; size 10 mm; size 5 mum; size 7 mum; three-dimensional imaging; two-dimensional en face subsurface histology mosaicing; Fluorescence; Humans; In vivo; Infrared imaging; Micromechanical devices; Microscopy; Optical imaging; Probes; Skin; Two dimensional displays;

fLanguage

English

Publisher

ieee

Conference_Titel

Optical MEMS and Nanophotonics, 2009 IEEE/LEOS International Conference on

Conference_Location

Clearwater, FL

Print_ISBN

978-1-4244-2382-8

Electronic_ISBN

978-1-4244-2382-8

Type

conf

DOI

10.1109/OMEMS.2009.5338633

Filename

5338633