Influences of probiotics to IL-7 mRNA expression in local mucosal tissues of gastrointestinal and respiratory of chickens

Author

Shao, Yue ; Zheng, Shi-min

Author_Institution

Coll. of Veterinary Med., Northeast Agric. Univ., Harbin, China

fYear

2011

fDate

24-26 June 2011

Firstpage

7645

Lastpage

7648

Abstract

According to the chicken´s IL-7 gene sequences available in GenBank, a specific primer pairs was designed by Primer. To appraisement and sequencing, the total RNA extraction in local mucosal tissues of gastrointestinal and respiratory of chickens after administration probiotics, the products were amplification by the RT-PCR. The levels of IL-7 mRNA expression were analyzed by real-time fluorescence quantitative PCR. As a result, the expression of IL-7 mRNA in local mucosal tissues of gastrointestinal and respiratory of chickens after administration probiotics was increased comparing with normal tissues.

Keywords

biological tissues; cellular biophysics; fluorescence; organic compounds; GenBank; IL-7 mRNA expression; RNA extraction; RT-PCR amplification; chicken; gastrointestinal system; gene sequences; local mucosal tissues; probiotics; real time fluorescence quantitative PCR; respiratory system; DNA; Fluorescence; Gastrointestinal tract; Glands; Immune system; RNA; Real time systems; chickens; expression of IL-7 mRNA; fluorescent quantitation PCR; probiotics;

fLanguage

English

Publisher

ieee

Conference_Titel

Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on

Conference_Location

Nanjing

Print_ISBN

978-1-4244-9172-8

Type

conf

DOI

10.1109/RSETE.2011.5966145

Filename

5966145