Spectrometric study on the interaction between peroxynitrite and Bovine Serum Albumin

Bovine Serum Albumin (BSA) was used as a target protein to study the structure change through tyrosine nitration by peroxynitrite. The correlation between BSA nitration and the structure change was established. The results show that the nitration of BSA by simulating the situation in vivo (continuous infusion of ONOO^-) is two times efficiency than infusion ONOO^- at one time. The nitration by peroxynitrite lead to α-helix structure reduced and β-sheet structure increased, which make significant changes of BSA backbone.