Segmentation and 3D Reconstruction of Microbial Biofilms

Author

Bouchet, A. ; Pastore, J.I. ; Di Meglio, L. ; Robuschi, L. ; Ballarin, V.

Volume

11

Issue

1

fYear

2013

fDate

Feb. 2013

Firstpage

324

Lastpage

328

Abstract

Capability of microbial biofilms to form biofilms is really important in biotechnology industries, environmental applications and medical applications. Therefore, the study of the structure and development of these organisms is essential. In this paper we develop and implement an algorithm, based on Mathematical Morphology, to analyze the structure and growing of microbial biofilms. This algorithm allows segmentation of a stack of images acquired from in vivo observation of microorganisms, using an inverted phase contrast microscope. Finally, a 3D reconstruction for an efficient visualization of the structure and topography of biofilms is done.

Keywords

biomedical materials; image reconstruction; image segmentation; medical image processing; microorganisms; thin films; 3D reconstruction; Mathematical Morphology; biofilm structure; biofilm topography; biotechnology industries; environmental applications; image segmentation; in vivo observation; inverted phase contrast microscope; medical applications; microbial biofilms; microorganisms; visualization; Image segmentation; In vivo; Microorganisms; Morphology; Three-dimensional displays; Visualization; 3D Reconstruction; Biofilms; Mathematical Morphology; Segmentation;

fLanguage

English

Journal_Title

Latin America Transactions, IEEE (Revista IEEE America Latina)

Publisher

ieee

ISSN

1548-0992

Type

jour

DOI

10.1109/TLA.2013.6502824

Filename

6502824