Characterization of anhydrous silanization and antibody immobilization on silicon dioxide surface

Author

Joshi, Madhura ; Goyal, M. ; Pinto, R. ; Mukherji, Soumyo

Author_Institution

Sch. of Biosci. & Bioeng., Indian Inst. of Technol. Bombay, Mumbai

fYear

0

fDate

0-0 0

Firstpage

7

Lastpage

11

Abstract

Formation of uniform and cluster free silane monolayer is one of the fundamental prerequisite for affinity cantilever based biosensors. We report anhydrous silanization protocol for uniform silane monolayer on silicon dioxide (SiO₂) surface using [3-(2-aminoethyl) aminopropyl)-trimethoxysilane (APTES) and characterized by AFM, spectroscopic ellipsometry and FTIR. Silanization coverage is controlled by availability of surface water. The roughness of the resulting silanized surface following our protocol is in the range of SiO₂ surface roughness. Silanized SiO₂ surface is used to immobilize human immunoglobulin (HIgG) on it. FITC tagged goat antihuman IgG is allowed to react with HIgG. The immobilized surface is further characterized using fluorescent spectroscopy and fluorescent microscope. Characterization results obtained from anhydrous silanization protocol are compared with the conventional aqueous silanization protocol

Keywords

Fourier transform spectra; atomic force microscopy; biosensors; cantilevers; ellipsometry; fluorescence; infrared spectra; molecular biophysics; optical microscopy; proteins; silicon compounds; surface roughness; surface treatment; AFM; FITC tagged goat antihuman IgG; FTIR; SiO₂; [3-(2-aminoethyl) aminopropyl)-trimethoxysilane; affinity cantilever based biosensors; anhydrous silanization; antibody immobilization; fluorescent microscope; fluorescent spectroscopy; human immunoglobulin immobilization; silicon dioxide surface; spectroscopic ellipsometry; surface roughness; uniform cluster free silane monolayer; Biosensors; Ellipsometry; Fluorescence; Humans; Immune system; Protocols; Rough surfaces; Silicon compounds; Spectroscopy; Surface roughness; APTES; FITC; affinity cantilever; silanization;

fLanguage

English

Publisher

ieee

Conference_Titel

Computer Architectures for Machine Perception, 2003 IEEE International Workshop on

Conference_Location

Hong Kong

Print_ISBN

0-7803-8612-4

Type

conf

DOI

10.1109/ISSMD.2004.1689550

Filename

1689550