Title :
Bio-MEMS sensors for real-time shear stress on endothelial cell dynamics
Author :
Hsiai, Tzung K. ; Cho, Sung K. ; Wong, Pak K. ; Ing, Michael ; Navab, Mohammad ; Reddy, Srinuvasa ; Demer, Linda L. ; Ho, Chih M.
Author_Institution :
Dept. of Biomed. Eng., Univ. of Southern California, Los Angeles, CA, USA
Abstract :
Precise characterization of shear stress in the arterial trees is critical to elucidate the effects of spatial versus temporal shear stress gradients on the biological activities of endothelial cells (EC). We developed micro electrical mechanical systems (MEMS) sensors, comparable to the size of EC (2 × 80 μm), to deliver the spatial and temporal resolution necessary at a frequency response > 100 Hz. We provided the first in vitro evidence of real-time wall shear stress on EC couple with real-time gene expression of monocyte chemoattractant protein (MCP-1).
Keywords :
biological techniques; blood vessels; cellular biophysics; frequency response; haemorheology; microsensors; proteins; 100 Hz; 80 micron; arterial trees; bio-MEMS sensors; biological activities; cell morphology regulation; endothelial cell dynamics; monocytes; real-time gene expression; real-time shear stress; spatial shear stress gradients; temporal shear stress gradients; Biosensors; Cells (biology); Mechanical sensors; Mechanical systems; Micromechanical devices; Real time systems; Sensor phenomena and characterization; Sensor systems; Spatial resolution; Stress;
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
Print_ISBN :
0-7803-7612-9
DOI :
10.1109/IEMBS.2002.1053047
