Wave shape classification of spontaneous neuronal activity in cortical cultures on micro-electrode arrays

Author

van Staveren, G.W. ; Buitenweg, J.R. ; Heida, T. ; Rutten, W.L.C.

Author_Institution

Dept. of Biomed. Eng., Twente Univ., Enschede, Netherlands

Volume

3

fYear

2002

fDate

23-26 Oct. 2002

Firstpage

2010

Abstract

Dissociated embryonal or postnatal rat cortical cells were cultured onto multi electrode arrays (MEA´s) with 61 electrode sites. They developed into networks and became spontaneously active after about one week in vitro. About 180,000 recorded action potential waveforms were sorted using several spike features and classified with a Mahalanobis distance sorting procedure. They were classified into six basic wave shapes.

Keywords

bioelectric potentials; biological techniques; brain; cellular biophysics; microelectrodes; neurophysiology; signal processing; 1 w; Mahalanobis distance sorting procedure; action potential waveforms; basic wave shapes; dissociated embryonal cells; multielectrode arrays; postnatal rat cortical cells; rat dissociated cortical cells; spike features; spontaneously active networks; Bioelectric phenomena; Biomedical electrodes; Biomedical engineering; Cells (biology); Embryo; In vitro; Neurons; Noise level; Scattering; Shape;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint

ISSN

1094-687X

Print_ISBN

0-7803-7612-9

Type

conf

DOI

10.1109/IEMBS.2002.1053141

Filename

1053141