Effects of cellular differentiation on autofluorescence emission from tissue engineered epidermis

Author

Smith, K. Given ; Relue, P.A.

Author_Institution

Dept. of Bioeng., Toledo Univ., OH, USA

Volume

3

fYear

2002

fDate

23-26 Oct. 2002

Firstpage

2299

Abstract

Malignant melanoma is the most lethal form of skin cancer, but one of the most successfully treatable if diagnosed early and accurately. Consequently, current research efforts are focused on developing an autofluorescence technique in early detection of malignant transformation of nevi. In order to understand the underlying mechanisms of light interaction with normal skin tissue, we have studied the fluorescence characteristics of tissue engineered pigmented epidermal models as they mature. Observed intensity variations with time are mapped to both the presence of endogenous fluorophores and chromophores within the tissue and the thickness of proliferating and differentiated cell layers. This mapping could be useful in the autofluorescence characterization of malignant tissues.

Keywords

biomedical optical imaging; cancer; cellular biophysics; fluorescence; skin; autofluorescence emission; cellular differentiation effects; differentiated cell layers; endogenous fluorophores; light interaction; light interaction mechanisms; malignant tissues characterization; nevi malignant transformation; proliferating cell layers; tissue engineered epidermis; tissue engineered pigmented epidermal models; Biomedical optical imaging; Cancer; Diseases; Epidermis; Fluorescence; Humans; Malignant tumors; Optical imaging; Optical scattering; Skin;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint

ISSN

1094-687X

Print_ISBN

0-7803-7612-9

Type

conf

DOI

10.1109/IEMBS.2002.1053291

Filename

1053291