Title :
4-Hydroxyphenylacetic Acid as a Monophenolase Inhibitor and a Diphenolase Activator on Mushroom Tyrosinase
Author :
Xie, Jian-Gang ; Li, Shu-Bai ; Xue, Yong ; Zhang, Hai-Tao ; Quan, Jing ; Nie, Hua-Li ; Zhu, Li-Min ; Branford-White, Christopher
Author_Institution :
Coll. of Chem., Chem. Eng. & Biotechnol., Donghua Univ., Shanghai, China
Abstract :
4-Hydroxyphenylacetic acid (4-HPA) can inhibit monophenolase (IC50=0.79 mM) and activate diphenolase of mushroom tyrosinase with its increasing concentrations. The activation kinetics, analyzed by Lineweaver-Burk plots, indicated 4-HPA to be a competitive and uncompetitive mixed-type activator of diphenolase when L-DOPA was used as substrate. 4-HPA can not only inhibit the oxy form but also activate the met form of mushroom tyrosinase.
Keywords :
biochemistry; enzymes; molecular biophysics; 4-hydroxyphenylacetic acid; Lineweaver-Burk plots; activation kinetics; diphenolase activator; monophenolase inhibitor; mushroom tyrosinase; Biochemistry; Chemicals; Chemistry; Educational institutions; Inhibitors; Insects; Kinetic theory; Optical polymers; Pharmaceuticals; Skin;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162144
