Rapid Detection of Adenine and Cytosine Nucleotides in Short Hetero-Oligodeoxynucleotides

Author

Mikelova, R. ; Trnkova, Libuse ; Jelen, Frantisek ; Hubalek, Jaromir ; Adam, Vojtech ; Kizek, Rene

Author_Institution

Masaryk Univ., Brno

fYear

2006

fDate

22-25 Oct. 2006

Firstpage

1175

Lastpage

1178

Abstract

Electrochemical methods are suitable techniques for study of oligodeoxynucleotides (ODNs) and their components. The combination of new techniques, the elimination voltammetry with linear scan (EVLS) and adsorptive stripping (AdS) and/or adsorptive transfer stripping (AdTS), was used for the rapid and low-cost detection of adenine and cytosine nucleotides in short synthetic hetero-ODNs. Using a mercury drop electrode and/or an amalgam electrode (AE) we are able not only to analyze ODNs at picomolar concentrations but also to resolve the adenine (A) and cytosine (C) nucleotide sequences in ODN chains.

Keywords

electrochemical electrodes; electrochemistry; molecular biophysics; organic compounds; adenine; adsorptive stripping; adsorptive transfer stripping; amalgam electrode; cytosine; electrochemical methods; elimination voltammetry; hetero-oligodeoxynucleotides; linear scan; mercury drop electrode; rapid detection; Biosensors; Chemistry; Chromium; DNA; Electrodes; Electrokinetics; Sequences; Signal analysis; Signal processing; Signal resolution;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors, 2006. 5th IEEE Conference on

Conference_Location

Daegu

ISSN

1930-0395

Print_ISBN

1-4244-0375-8

Electronic_ISBN

1930-0395

Type

conf

DOI

10.1109/ICSENS.2007.355828

Filename

4178823