Title :
Analysis of Seven Polyacrylamide(PAM)-Degrading Bacterias Using PCR-DGGE Method
Author :
She, Yuehui ; Zhang, Fan ; Wang, Zhengliang ; Zhao, Liping ; Yu, Longjiang
Author_Institution :
Sch. of Chem. & Environ. Eng., Yangtze Univ., Jingzhou, China
Abstract :
Seven polyacrylamide(PAM)-degrading bacteria were isolated from waste water treated pool of Henan(P.R.China) oil field, six of which were isolated from the sample of sludge, one from the sample of waste water. Sequences and phylogenetic analysis of 16 S rDNA clones of the seven bacteria showed that four were affiliated with Actinobacteria, two with Alphaproteobacteria, one with Bacillus. PCR-DGGE profile was used to analyze the environmental dominant status of the seven bacteria. DGGE bands of five bacteria can be found in the lane of DNA from original water or sludge samples. The result provided valuable information about PAM waste treatment of He Nan Henan oil field.
Keywords :
biochemistry; microorganisms; sludge treatment; wastewater treatment; Actinobacteria; Alphaproteobacteria; Bacillus sp; Henan oil field wastewater pool; PAM degrading bacteria; PCR-DGGE method; phylogenetic analysis; polyacrylamide degrading bacteria; sludge; Chemical technology; DNA; Degradation; Filters; Microorganisms; Petroleum; Phylogeny; Sequences; Sludge treatment; Wastewater treatment;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162356
