Title :
Analysis of SSR Marker of Wheat Mutants of Genetic Transformation Mediated by Ion Beam
Author :
Wang, Tie-gu ; Li, Xue-bin ; Shi, Ming-wang ; Wang, Wei-dong ; Chen, Shi-lin ; Ou, Xing-qi ; Liu, Ming-jiu
Author_Institution :
Henan Inst. of Sci. & Technol., Xinxiang, China
Abstract :
13 lines including 9 wheat mutants of genetic transformation by ion beam and 4 acceptor control varieties were analyzed by SSR marker. A special band of dwarf mutant line 1042 was obtained, sequenced and analyzed by blast software. The genetic distance was analyzed with SSR marker materials by SAS software. The result showed that 9 wheat mutants were clustered together with their own acceptor control varieties respectively. These suggested that the variance of mutants of genetic transformation mediated by ion beam was smaller than genetic variance among different varieties. The result aligned by blast software showed that the special sequence of dwarf mutant line 1042 was a part of the Rht-D1a gene. These suggested that the reason of declining the height of 1042 mutant line was Rht-D1a mutant.
Keywords :
DNA; biochemistry; biological effects of ionising particles; biological techniques; cellular biophysics; electrophoresis; genetics; ion beam effects; molecular biophysics; Rht-D1a mutant; SAS software; SSR marker; acceptor control variety; blast software; clustering; dwarf mutant line sequence; genetic distance; genetic transformation; ion beam; wheat mutants; Biological materials; Biomedical engineering; Crops; DNA; Genetics; Ion beams; Laboratories; Proteins; Sequential analysis; Synthetic aperture sonar;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162357
