Author_Institution :
State Key Lab. of Pollution Control & Resource Reuse, Tongji Univ., Shanghai, China
Abstract :
Microbial community compositions and functional activities of three acid mine drainage (AMD) sediment samples from different sites in Dexing copper mine were analyzed by 50- mer oligonucleotide arrays which have been constructed and evaluated. The results showed that microbial community was composed of 15 different genus or species, including Acidithiobacillus ferrooxidans(A. ferrooxidans), Leptospirillum sp., Acidiphilum spp., Acidithiobacillus thiooxidans, Acidobacteria, Acidithiobacillus caldus, Sulfobacillus sp., Desulfovibrio longus, Syntrophus sp., metallosphaera, Alicyclobacillus spp., Holophaga sp., Sulfolobus sp., Thiomonas spp. and Actinomyces naeslundii. Acidiphilum was abundant in three samples, for the anaerobic environment of sediment could inhibited aerobic bacteria, such as A. ferrooxidans. Heterotrophic Acidiphilium is able to catalyze the dissimilatory reduction of ferric iron to ferrous even in the presence of small amounts of oxygen. Among all the samples, the diversity was high in samples (site YTW and ZJ) containing low pH while the diversity was low in sample (site KZX) containing very high pH. Microarray hybridization data indicated that iron metabolism functional gene, Sulfur metabolism functional gene and metal-resistance gene and other genes existed in the AMD system. At all samples examined, microbial community and function activities were positively correlated with pH.
Keywords :
biochemistry; catalysis; cellular biophysics; contamination; genetics; microorganisms; mining; molecular biophysics; pH; sediments; water pollution; 50-mer oligonucleotide array hybridization; A. ferrooxidans; Acidiphilum spp.; Acidithiobacillus caldus; Acidithiobacillus ferrooxidans; Acidithiobacillus thiooxidans; Actinomyces naeslundii; Alicyclobacillus spp.; Desulfovibrio longus; Dexing copper mine; Holophaga sp.; Leptospirillum sp.; Sulfobacillus sp.; Sulfolobus sp.; Syntrophus sp.; Thiomonas spp; acid mine drainage sediment; acidobacteria; aerobic bacteria; anaerobic environment; catalysis; dissimilatory reduction; ferric iron; heterotrophic acidiphilium; iron metabolism functional gene; metal-resistance gene; metallosphaera; microarray hybridization; microbial community profiling; oxygen amounts; pH; sulfur metabolism functional gene; Biochemistry; Copper; DNA; Iron; Microorganisms; Sediments; Surface contamination; Surface treatment; Water pollution; Water resources;
