cDNA Cloning and Bioinformatic Analysis of Self-Incompatible S34-Allele from Chinese Pears

Author

Zeng, Yanling ; Tan, Xiaofeng ; Zhang, Dangquan ; Hu, Xiaoyi ; Chen, Hongpeng ; Yuan, Deyi ; Zhang, Lin

Author_Institution

Non-wood Forest Products of State Forestry Adm., Central South Univ. of Forestry & Technol., Changsha, China

fYear

2009

fDate

11-13 June 2009

Firstpage

1

Lastpage

4

Abstract

The full-length cDNA of a new S-RNase (S ₃₄-RNase) (GenBank accession No.DQ414813) was successfully cloned from pistils of Chinese pear by RT-PCR and RACE methods. The S ₃₄-RNase shows a complete CDS of 687 nucleotides for putative 229 amino acids. The theoretical three-dimension structure of S ₃₄-RNase was predicted by homology modeling method and its structural characteristic was analyzed. Aligning of S ₃₄-RNase with other 39 S-RNases of pears revealed the question about unifying naming system.

Keywords

DNA; bioinformatics; botany; genetics; molecular biophysics; Chinese pear pistils; RACE method; RT-PCR method; S₃₄-RNase 3D structure; amino acid; bioinformatic analysis; cDNA cloning; full-length cDNA; homology modeling method; nucleotides; self-incompatible S₃₄-allele; unifying naming system; Amino acids; Bioinformatics; Biological materials; Cloning; Computer aided analysis; Forestry; Nitrogen; Plants (biology); Predictive models; RNA;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-2901-1

Electronic_ISBN

978-1-4244-2902-8

Type

conf

DOI

10.1109/ICBBE.2009.5162479

Filename

5162479