Cloning and Expression Analysis of Wild Boar Osteopontin Gene

Author

Dong-Jie Zhang ; Di Liu

Author_Institution

HeiLongJiang Acad. of Agric. Sci., Harbin, China

fYear

2009

fDate

11-13 June 2009

Firstpage

1

Lastpage

4

Abstract

The wild boar OPN cDNA in size of 909bp was cloned by RT-PCR and analyzed by bioinformatics. The result showed that wild boar OPN contains 1) a potential calcium phosphate apatite binding region of eight consecutive Asp residues beginning at amino acid 86, 2) a cell-attachment Gly-Arg-Gly- Asp-Ser (GRGDS) sequence (residues 153-157), 3) a thrombin RS cleavage site (residues 163 and 164). The phylogenetic tree showed that the relationship between wild boar and domestic pig was the most close, but farthest with zebrafish, OPN gene accord with evolution relation. Wild boar OPN mRNA was abroad expressed in different tissue, higher in heart, stomach, lung, muscle, uterus, and lower in liver, kidney, small intestine and ovary. The protein weight was different in different tissue 70kDa, 70kDa and 45kDa, 70kDa, 45kDa and 24kDa.

Keywords

DNA; bioinformatics; genetic engineering; genetics; genomics; molecular biophysics; proteins; Asp residues; Gly-Arg-Gly-Asp-Ser sequence; RT-PCR; amino acid; bioinformatics; calcium phosphate apatite binding region; cell attachment; cloning; domestic pig; expression analysis; heart; kidney; liver; lung; mRNA; muscle; ovary; phylogenetic tree; small intestine; stomach; thrombin RS cleavage site; uterus; wild boar OPN cDNA; wild boar osteopontin gene; zebrafish; Amino acids; Application specific processors; Bioinformatics; Calcium; Cloning; Heart; Lungs; Muscles; Phylogeny; Stomach;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-2901-1

Electronic_ISBN

978-1-4244-2902-8

Type

conf

DOI

10.1109/ICBBE.2009.5162510

Filename

5162510