Drug Release Properties of Poly (D, L-Lactic Acid) PDLLA Modified Genipin Cross-Linked Chitosan/Gelatin Scaffold

In the present study, poly (D, L-lactic acid) (PDLLA) modified genipin-cross-linked chitosan/gelatin scaffolds loaded icariin were prepared. The scaffolds with genipin cross-linking were fabricated with lyophilization, and then were immersed into the 2.5%, 5.0%, and 7.5% PDLLA-dichloromethane solutions. Thus the PDLLA modified genipin-cross-linked scaffolds were obtained after evaporating the solvent dichloromethane. The water absorption capacity and degradation rate of the scaffolds were measured and evaluated. The released icariin was determined with High Performance Liquid Chromatography (HPLC) method. The swelling ratio and degradation rate decreased significantly after the PDLLA modification. The loaded icariin released gradually in 9 days reaching to the maximum of 6.137, 4.530, 3.745, and 4.13 mg/g, respectively. The XTT assay was applied to evaluate the cytotoxicity and biocompatibility of the scaffolds with MC3T3-E1 osteoblast-like cells in this study.