Dynamic Monitoring of PEG-Mediated Cell Fusion by RT-CES System

Author

Deng, Huihua ; Guo, Airong ; Qian, Mengdie ; Liu, Xuan ; Zhou, Dingxin ; Tang, Zuming ; Lu, Zuhong

Author_Institution

Key Lab. of Child Dev. & Learning Sci. (SEU), Southeast Univ., Nanjing, China

fYear

2009

fDate

11-13 June 2009

Firstpage

1

Lastpage

4

Abstract

The response of MCF7 and A549 cells to the PEG treatment was monitored in the terms of impedance-related cell index curves by RT-CES system. The treatment of 50% PEG to MCF7 and A549 cells led to the one-stage drop of cell index with the culture time. Longer duration of PEG action to cells shortened the net detachment duration of cells from the surface of the electrode. The treatment of 15% PEG to MCF cells resulted in two-stage drop of cell index. These were attributed to PEG-mediated cell fusion, which was supported by SEM results.

Keywords

bioelectric phenomena; biomedical measurement; biosensors; cellular biophysics; electric impedance; scanning electron microscopy; A549 cell response; MCF7 cell response; PEG-mediated cell fusion; RT-CES system; SEM; cell detachment duration; cell-based biosensor; dynamic monitoring; impedance-related cell index curves; real-time cell electronic sensing system; Biology; Biosensors; Cells (biology); Electric variables measurement; Electrodes; Impedance measurement; Monitoring; Real time systems; Sensor arrays; Surface morphology;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-2901-1

Electronic_ISBN

978-1-4244-2902-8

Type

conf

DOI

10.1109/ICBBE.2009.5162668

Filename

5162668