Title :
Effect of Exogenous Cholesterol on Cholesterol Accumulation and mRNA Expression of SREBP-2 and HMGR in Goose Primary Hepatocytes
Author :
Han Chun-Chun ; Wang Ji-Wen ; Li, Wang ; Zhang Zhong-Xian ; Wang Li
Author_Institution :
Key Lab. of Animal Genetic Resources, Sichuan Agric. Univ., Ya´´an, China
Abstract :
In order to understand the effect of exogenous cholesterol on cholesterol accumulation and mRNA expression of SREBP-2 and HMGR in goose, primary hepatocytes from Landes Goose (Anser anser) and Tianfu Meat Goose (Anser cygnoides) were isolated and treated with the exogenous cholesterol. Cholesterol accumulation, and gene expression levels of SREBP-2 and HMGR were measured. The results indicated exogenous cholesterol cultured with hepatocytes for 72 h had no evident effect on TC content in hepatocytes. We found TC content increased before 24 h, decreased after 48 h. Exogenous cholesterol could decrease the mRNA level of SREBP-2 and HMGR. The correlation analysis indicated the mRNA expression of SREBP-2 was positively correlated to that of HMGR. In conclusion, exogenous cholesterol could regulate the cholesterol content by decreasing the gene expression of SREBP-2 and HMGR in goose primary hepatocytes.
Keywords :
biological techniques; cellular biophysics; correlation methods; enzymes; genetics; macromolecules; molecular biophysics; proteins; HMGR; SREBP-2; cholesterol accumulation; correlation analysis; exogenous cholesterol; gene expression; goose primary hepatocyte; landes goose; mRNA expression; tianfu meat goose; time 72 h; Animals; Biochemistry; Cells (biology); Feedback; Gene expression; Genetics; Lipidomics; Proteins; RNA; Real time systems;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162694
