Title :
Effects of Di-(2-Ethylhexyl) Phthalate on Root Tips of Vicia faba Seedlings
Author :
Chang Qing ; Gao Nana ; Zheng Yuduo ; Ding Shumao
Author_Institution :
Hubei Key Lab. of Genetic Regul. & Integrative Biol., Huazhong Normal Univ., Wuhan, China
Abstract :
In order to explore the effects of di-(2-ethylhexyl) phthalate (DEHP) on Seed and growth of seedling of Vicia faba. Root-tip of Vicia faba was exposed to different concentrations (0.01, 0.1, 1, 10 mgldrmL-1) of DEHP, it was carried out to investigate the effects of DEHP on micronucleus (MCN) rate and DNA polymorphism of Vicia faba. The results showed that micronucleus rate of Vicia faba increased with DEHP concentrations. The random amplified polymorphic DNA profiles showed obvious differences between normal and exposed plantlets, indicating a does - effect relationship. These results suggested that DEHP could induce the acute oxidative damage and genotoxic effects in growth of seedling of Vicia faba.
Keywords :
DNA; environmental factors; genomics; molecular biophysics; oxidation; polymorphism; toxicology; DNA polymorphism; Vicia faba seedlings; acute oxidative damage; di-(2-ethylhexyl) phthalate; genotoxic effects; micronucleus rate; plantlets; Bioinformatics; Chemical analysis; Crops; DNA; Genetic mutations; Genomics; Polymers; Production; Protocols; Soil;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162735
