Modelling Root Development with Signalling Control: A Case Study Based on Legume Autoregulation of Nodulation

In legume plants, the balance of nodule formation is maintained by a long-distance shoot-root signalling system known as autoregulation of nodulation. Due to the complexity of plants´ signalling nature, the underlying mechanisms of autoregulation of nodulation still remain largely unknown. The purpose of our research is to use functional-structural plant modelling to help in further investigating this regulatory system. For such a purpose, there are two major challenges to be met: modelling the 3D architecture of legume roots and coordinating signalling-development processes with various rates. Using soybean as the target plant and L-studio as the modelling platform, we have developed a series of methods and techniques to collect root development data, reconstruct root architecture, and synchronise the multi-rate signalling and developmental events. These methods and techniques may also be applicable to studies on lateral root initiation and relevant regulations.