Title :
Genotoxic Effects of Microcystin-LR in Mammalian Cells
Author :
Huang, Pei ; Xu, An
Author_Institution :
Key Lab. of Ion Beam Bioeng., CAS, Hefei, China
Abstract :
Microcystin-LR (MC-LR) is a heptapeptidic toxin produced by cyanobacteria. However, the genotoxic effects of MC-LR are still contrary. This study is focused on the DNA damage induced by MC-LR in human-hamster hybrid AL cell line, which is sensitive to various toxic factors. Our present data showed that MC-LR at a concentration of 0.1 mug/ml had slight effect on the celluar ATP contents and the colony formation of AL cells with either 1-, 3-, 7-, 15- or 30-day treatment. However, with 30-day treatment, the micronucleus frequency with 0.1 mug/ml was increased about 1.6-fold compared to the negative control. These data suggested that MC-LR was genotoxic to mammalian cells with minimal cytotoxicity.
Keywords :
DNA; biochemistry; cellular biophysics; genetics; microorganisms; molecular biophysics; proteins; toxicology; DNA damage; MC-LR; celluar ATP content; colony formation; cyanobacteria; cytotoxicity; genotoxic effect; heptapeptidic toxin; human-hamster hybrid AL cell line; mammalian cells; microcystin-LR; micronucleus frequency; Biological cells; Biomedical engineering; Cells (biology); Content addressable storage; DNA; Humans; Ion beams; Neoplasms; Physics; Plasma measurements;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162883
