Title :
Hyperosmotic Stress Induced Actin Filaments Varying in Arabidopsis thaliana Suspension Cells
Author :
Shi, Lanchun ; Wang, Bochu ; Gong, Wei ; Wang, YiChuan
Author_Institution :
Coll. of Bioeng., Chongqing Univ., Chongqing, China
Abstract :
With the suspension cells of Arabidopsis thaliana stably expressing the GFP-fimbrin1 fusion protein and confocal microscope, the actin filaments distribution of single cell was investigated in real-time during hyperosmotic exposure. Our study suggested that the maintenance and recovery of normal cell volume is related with actin cytoskeleton, and the reorganization of AFs is following the shrinkage of plasmalemma during plasmolytic process.
Keywords :
biological techniques; biomembranes; cellular biophysics; optical microscopy; proteins; Arabidopsis thaliana suspension cell; GFP-fimbrin1 fusion protein; actin cytoskeleton; confocal microscope; hyperosmotic stress; plasmalemma shrinkage; plasmolytic process; Animals; Biomedical engineering; Educational institutions; Fluorescence; Genetics; Microscopy; Plasma materials processing; Proteins; Stress; Visualization;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162912
