Inhibitive Effects of Lipoic Acid on the DNA-Protein Crosslink Induced by Formaldehyde in Mice Liver

Author

Liang Si-Si ; Cao Yi ; Kun Wang ; Fu Cong ; Xiao Lin ; Yang Xu

Author_Institution

Hubei Key Lab. of Genetic Regul. & Integrative Biol., Huazhong Normal Univ., Wuhan, China

fYear

2009

fDate

11-13 June 2009

Firstpage

1

Lastpage

4

Abstract

The objective of this study is to explore the relation between oxidative stress and DNA-protein crosslink formation caused by formaldehyde (FA) inhalation in mice liver. 15 Kun Ming mice were divided into 3 groups: control group, 3.0 mg/m³ gaseous FA inhaled group, 3.0 mg/m³ gaseous FA and lipoic acid (LA) treated group. The content of reduced glutathione (GSH) and the content of DPC were measured after the treatment for 3d. The content of GSH in liver cells declined significantly and the content of DPC increased significantly after inhaled with 3.0 mg/m³ gaseous FA. The content of GSH and DPC were both recovered to control group after treated with 3.0 mg/m³ gaseous FA inhalation and LA injection. LA injection could inhibit the formation of DPC in mice liver after exposure to FA. It is suggested that DPC formation in mice liver may be related with oxidative damage.

Keywords

DNA; biological techniques; cellular biophysics; liver; molecular biophysics; proteins; DNA-protein crosslink; DPC measurement; formaldehyde inhalation; glutathione; lipoic acid; liver cell; oxidative damage; oxidative stress; Air pollution; Fluorescence; Genetics; Glass; Humidity; Liver; Mice; Stress; Temperature sensors; Ventilation;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-2901-1

Electronic_ISBN

978-1-4244-2902-8

Type

conf

DOI

10.1109/ICBBE.2009.5162971

Filename

5162971