Author :
Shi, Ming-wang ; Wang, Qing-lian ; Deng, Tian-fu ; Ou, Xing-qi ; Li, Xue-bin ; Liu, Qiu-yun ; Li, Xue-yong ; Lang, Jian-feng
Abstract :
Lipoxygenases are non-heme iron-containing dioxygenases that are widely distributed in plants. Construction of cDNA Library and cloning of wild soybean seed lipoxygenase gene LOX, the structural characteristics of the gene expression and enzyme characteristics were analyzed. Bioinformatics analysis showed that the N-terminal contains about 60 additional amino acids of LOX, LOX may have activity. Lox and soybean gene with the highest cDNA sequences (about 79%), Phaseolus vulgaris, Arachis hypogaea, Glycine max, Solanum tuberosum, Solanum tuberosum, Solanum tuberosum, Prunus dulcis Miller., Prunus armeniaca, Prunus armeniaca, Pyrus pyrifolia, Persea americana, Arabidopsis thaliana, Adelostemma gracillimum the cDNA sequences were 76,76,79,77,71,70,70,71,69,70,69,67,67 and 70%, respectively.
Keywords :
DNA; bioinformatics; botany; genomics; molecular configurations; Adelostemma gracillimum; Arabidopsis thaliana; Arachis hypogaea; Glycine max; LOX N-terminal; LOX amino acids; LOX cloning; LOX gene functional analysis; LOX gene isolation; Persea americana; Phaseolus vulgaris; Prunus armeniaca; Prunus dulcis; Pyrus pyrifolia; Solanum tuberosum; bioinformatic analysis; cDNA library construction; enzyme characteristics; gene expression structural characteristics; lipoxygenases; nonheme iron containing dioxygenases; wild soybean seeds; Amino acids; Bioinformatics; Cloning; Functional analysis; Immune system; Libraries; Lipidomics; Pathogens; RNA; Surface resistance;
