Isotherm, Kinetic and Thermodynamic Analysis of Bromelain Adsorption on Reactive Blue 4 Immobilized Composite Membranes

The behavior of bromelain adsorption on the dye- ligand affinity membrane was investigated at 277,298 and 310 K, which was chitosan (CS) coated to decrease the non-specific adsorption of protein. Reactive Blue 4 was immobilized onto the CS-coated nylon membrane as an affinity ligand. In order to examine the rate limiting step of Reactive Blue 4-immobilized composite membrane adsorption, the two adsorption kinetic model: the pseudo-first-order and the pseudo-second-order were tested with the experiment data. Results indicated that Freundlich isotherm was able to adequately describe the adsorption of bromelain on dye affinity membranes. It was also found that the pseudo-first-order kinetic model described the bromelain adsorption process with a good fitting.