Title :
Kinetic Analysis of Aminoethylisothiourea on Diphenolase of Mushroom Tyrosinase
Author :
Li, Shu-Bai ; Zhang, Hai-Tao ; Xue, Yong ; Nie, Hua-Li ; Zhu, Li-Min ; Zhang, Xiao-Fei ; Branford-White, Christopher
Author_Institution :
Coll. of Chem., Chem. Eng. & Biotechnol., Donghua Univ., Shanghai, China
Abstract :
This study demonstrates that Aminoethylisothiourea (AET), a potent inhibitor of inducible nitric oxide synthase (NOS), is an irreversible competitive inhibitor of the diphenolase activity of mushroom tyrosinase when L-DOPA was assayed spectrophotometrically in vitro. The inhibition of diphenolase activity of tyrosinase by AET showed positive kinetic cooperativity which arose from the protection of both substrate and o-quinone against inhibition by AET.
Keywords :
biochemistry; enzymes; molecular biophysics; reaction kinetics; spectrophotometry; aminoethylisothiourea; diphenolase; inducible nitric oxide synthase; inhibitor; kinetic analysis; mushroom tyrosinase; o-quinone; spectrophotometry; Biochemistry; Copper; Data analysis; Drugs; Inhibitors; Kinetic theory; Nonlinear equations; Proteins; Switches; Variable structure systems;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5163003
