Microstructured Topography Enhanced the Responsiveness of Voltage-Gated Calcium Channels in H945RB.3 Human Neural Progenitor Cells

Author

Wu, Ze-Zhi ; Kisaalita, William S. ; Wang, Lina ; Zhao, Yiping

Author_Institution

Coll. of Bioeng., Chongqing Univ., Chongqing, China

fYear

2009

fDate

11-13 June 2009

Firstpage

1

Lastpage

4

Abstract

A topographical substrate with a packed polystyrene microbead (1.98 plusmn 0.20 mum in diameter) array was fabricated for the development of cell-based assay systems targeting voltage-gated calcium channels (VGCCs). We found that the microbead arrayed substrates enhanced the attachment and spreading of the cultured human neural progenitor cells (H945RB.3) as compared to the flat polystyrene surfaces. Microbead arrayed substrates also facilitated the development of higher VGCC responsiveness upon neuronal differentiation than flat substrates. The enhancement of both VGCC responsiveness and cell spreading were most significant until day 14 into differentiation.

Keywords

bioelectric phenomena; biomembrane transport; calcium; microfabrication; neurophysiology; polymers; surface topography; H945RB.3 human neural progenitor cell; cell differentiation; cell spreading; cell-based assay system; microstructured topography; neuronal differentiation; polystyrene microbead array; polystyrene surface; surface topography; voltage-gated calcium channel; Biomembranes; Calcium; Cells (biology); Delay; Fluorescence; Humans; Scanning electron microscopy; Surface morphology; Surface topography; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-2901-1

Electronic_ISBN

978-1-4244-2902-8

Type

conf

DOI

10.1109/ICBBE.2009.5163055

Filename

5163055