Purification of Microcystin-LR by Solid-Phase Extraction Procedure

Author

Chen, Xiaoguo ; Zhang, Shenghu ; Zhang, Yang ; Xiao, Bangding

Author_Institution

Coll. of Resources & Environ. Eng., Wuhan Univ. of Technol., Wuhan, China

fYear

2009

fDate

11-13 June 2009

Firstpage

1

Lastpage

4

Abstract

Solid-phase extraction procedure was successfully used to purify microcystin-LR from cultured cyanobacteria. The method involved collection of cells by flocculation with polyaluminium chloride, extraction of harvested cells by 50% aqueous methanol, clean-up by liquid-liquid extraction with dichloromethane, and purification by solid-phase extraction. More than 8 mg of microcystin-LR with a purity of 95% (at 238 nm) was obtained in a single run (recovery, 85%). The present method provides a simple and efficient one step purification eliminating the need to use preparative HPLC.

Keywords

biological techniques; cellular biophysics; flocculation; microorganisms; organic compounds; purification; aqueous methanol; cell extraction; cyanobacteria; dichloromethane; flocculation method; liquid-liquid extraction clean-up; microcystin-LR purification; polyaluminium chloride; solid-phase extraction procedure; Amino acids; Calibration; Educational institutions; Impurities; Lead; Mass spectroscopy; Methanol; Proteins; Purification; Solvents;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-2901-1

Electronic_ISBN

978-1-4244-2902-8

Type

conf

DOI

10.1109/ICBBE.2009.5163264

Filename

5163264