Title :
PRECISE LOCALIZATION OF FLUORESCENT PROBES WITHOUT NUMERICAL FITTING
Author :
Andersson, Sean B.
Author_Institution :
Aerosp. & Mech. Eng., Boston Univ., MA
Abstract :
A new algorithm for determining the location of a fluorescent probe in the plane is described. Inspired by the Bancroft algorithm for localization in the global positioning system, the technique calculates the position of the probe without the use of a numerical fitting procedure. Numerical simulations are presented which indicate that the precision of the localization is similar to that obtained using the standard method of fitting a Gaussian profile to the measured fluorescence intensity. Moreover, the computational time is two orders of magnitude shorter than when performing such a fit.
Keywords :
biomedical imaging; fluorescence; optical microscopy; probes; Bancroft algorithm; Gaussian profile; fluorescent probes; global positioning system; Background noise; Biomedical measurements; Charge coupled devices; Current measurement; Diffraction; Fluorescence; Microscopy; Position measurement; Probes; Wavelength measurement;
Conference_Titel :
Biomedical Imaging: From Nano to Macro, 2007. ISBI 2007. 4th IEEE International Symposium on
Conference_Location :
Arlington, VA
Print_ISBN :
1-4244-0672-2
Electronic_ISBN :
1-4244-0672-2
DOI :
10.1109/ISBI.2007.356836
