Title :
Simultaneous Determination of Five Water-Soluble Components of Danshen in Rabbit Plasma and Its Application to Pharmacokinetics
Author :
Yuan, Jin-Chan ; Yao, Ji-Hong ; Han, Guo-Zhu ; Hu, Xiao-Wei
Author_Institution :
Zhongshan Coll., Dalian Med. Univ., Dalian, China
Abstract :
A sensitive and specific high-performance liquid chromatography (HPLC)-UV method has been developed for the simultaneous determination of five water-soluble components, Danshensu (DSS), protocatechuic acid (PAC), protocatechuic aldehyde (PAL), salvianolic acid B (SAB) and salvianolic acid A (SAA) of Danshen Injection in rabbits. After liquid-liquid extraction with ethyl acetate, plasma sample was eluted from CAP CELL PAK C18 column with a gradient mobile phase composed of component A: water- dimethylformamide-acetic acid (94:4:2) and B: methanol- dimethyl formamide-acetic acid (94:4:2). The detection wavelength was set at 281 nm and flow rate was 1.0 mL/lmin. The method described in the present paper showed good linearity over a relative wide range and recoveries of 70.3-94.7%, as well as high intra- and inter-day precisions (CV <15%). The limit of detection for DSS , PAC, PAL, SAB and SAA in plasma were 0.1, 0.05, 0.05, 0.1, 0.2 mug/mL, respectively. It could be concluded that this method is sensitive, specific, reproducible and completely meet requirements for pharmacokinetic study of five water-soluble components after intravenous administration of Danshen Injection.
Keywords :
biochemistry; chromatography; organic compounds; pharmaceuticals; solubility; CAP CELL PAK C18 column; Danshen injection; Danshensu; HPLC-UV method; ethyl acetate; high-performance liquid chromatography; liquid-liquid extraction; methanol-dimethyl formamide-acetic acid; pharmacokinetics; protocatechuic acid; protocatechuic aldehyde; rabbit plasma; salvianolic acid A; salvianolic acid B; water-dimethyl formamide-acetic acid; water-soluble components; Animals; Cardiac disease; Chemical analysis; Decision support systems; Ischemic pain; Myocardium; Pharmaceuticals; Plasma applications; Protection; Rabbits;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5163403