Mining osmotic stress response genes from Arabidopsis genome

The five-chromosome sequence files of Arabidopsis thaliana, which included huge amounts of genetic sequences and annotation information. How to get the tap osmotic stress response gene is already unable to meet the needs of these algorithms. Based on analysis of plant genes´ interaction principle, a reasonable technological line has been presented in this paper. First, we establish a basic function base in order to offer kinds of convenient tool functions. Then we produce index files of mRNA and gene sequence to predigest GenBank flat files and improve efficiency. We can get the range of promoters combining with predicted TSS. Finally, we design a reasonable algorithm to calculate P value. Hereby researchers can conclude if the cis element correlates with osmotic stress when it appears in promoter region, which can provide infromation for reachers to carry on the biological information scientific and the molecular biology aspect reserch.