Title :
Local microinjection near nerve axon of C. elegans with hydro-gel beads based on micromanipulation
Author :
Nakajima, M. ; Ayamura, Y. ; Takeuchi, M. ; Hisamoto, N. ; Strahil, P. ; Hasegawa, Y. ; Fukuda, T.
Author_Institution :
Center For Micro-nano Mechatron. of Nagoya Univ., Nagoya, Japan
Abstract :
This paper presents a local microinjection system using hydro-gel beads based on micromanipulation near nerve axon of Caenorhabditis elegans (C. elegans), which is one of the important model organism for disease analysis and nerve analysis. The hydro-gel beads were prepared using Polyethylene Glycol Diacrylate (PEG-DA) to encapsulate biomaterials. The tip of micro-pipette tool was used to attach the hydro-gel beads by a negative pressure with a syringe. The local-nano-injection technique was demonstrated to inject fluorescent materials into C. elegans using the micro-pipette tool by a piezo actuator. The microinjection was evaluated in a three dimensional space by a confocal microscope. The proposed microinjection method is considered to be important as future micro-nano surgery system for life innovation using model organism.
Keywords :
biomedical equipment; biomedical materials; biomedical optical imaging; cellular biophysics; diseases; encapsulation; fluorescence; high-speed optical techniques; hydrogels; microorganisms; neurophysiology; optical microscopy; C. elegans; Caenorhabditis elegans; biomaterials; confocal microscopy; disease analysis; encapsulaion; fluorescent materials; hydrogel beads; local microinjection near nerve axon; micromanipulation near nerve axon; micronano surgery system; micropipette tool; model organism; nerve analysis; piezoactuator; polyethylene glycol diacrylate; three dimensional space; Educational institutions;
Conference_Titel :
System Integration (SII), 2014 IEEE/SICE International Symposium on
Conference_Location :
Tokyo
Print_ISBN :
978-1-4799-6942-5
DOI :
10.1109/SII.2014.7028014