Study on the Mechanism of Oxidative Damage and Genotoxicity induced by BBP in Hepatic cells of Mice

To illustrate the possible function of genotoxicity of BBP on DNA, this study took mice livers as experimental materials to detect the levels of DNA-protein crosslinks (DPC), the activity of surperoxide dismutase (SOD), and the contents of malondialdehyde (MDA) with intraperitioneal injection of different doses of BBP for two consecutive 14 days. DPC levels in hepatic cells showed a dose-dependent enhancement after administration of BBP. Significant DPC was observed in the 500 and 1000 mg/(kgldrd) (P<0.01) groups, compared to the control group. A decrease in the activities of SOD, companied by a increase in the MDA contens, was observed. Significant changes of SOD activity could be observed in 250, 500 and 1000 mg/(kgldrd) (P<0.01) groups. When the exposure doses were 250, 500 and 1000 mg/(kgldrd), there were dramatic changes in the levels of MDA (P<0.01). To conclusion, high levels BBP induced significant oxidative damage, then caused damage to DNA.