The Adsorption of Protein Molecules at a Crystal/solution Interface Observed by an Improved TIRFM

Author

Dai, Guoliang ; Sazaki, Gen

Author_Institution

Nat. Microgravity Lab., Chinese Acad. of Sci., Beijing, China

fYear

2009

fDate

11-13 June 2009

Firstpage

1

Lastpage

3

Abstract

We have improved the ordinary total internal reflection fluorescence microscopy (TIRFM). Two improvements have been achieved, one is the interface between opaque material and solution can be observed, another is the interface far away (usually several ten micro meters) the objective lens can be observed. By this improved TIRFM, the adsorption of protein molecules at a crystal/solution interface had been successfully observed. We have obtained the results of relationship between the amount of adsorbed protein molecules on bunched steps and the height of bunched steps of a protein crystal.

Keywords

adsorption; bio-optics; biological techniques; fluorescence; molecular biophysics; optical microscopy; proteins; TIRFM; crystal-solution interface; objective lens; opaque material; protein molecule adsorption; total internal reflection fluorescence microscopy; Chemicals; Crystalline materials; Fluorescence; Laser beams; Lenses; Microscopy; Optical materials; Optical reflection; Proteins; Solids;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-2901-1

Electronic_ISBN

978-1-4244-2902-8

Type

conf

DOI

10.1109/ICBBE.2009.5163569

Filename

5163569