Dynamic attachment of HepG2 in fractal microchannels

Dynamic filling experiments using HepG2 tumor cells are performed for observing the attachment behavior novelly in fractal microchannels. A PDMS microchannel mimicking the capillary blood vessel with fractal configuration is fabricated by the gelatin salt-out technique and the PDMS soft lithography. The fabrication of this low-toxicity fractal PDMS microchannel is firstly addressed herein. Using this PDMS microchannel, PBS with living HepG2 tumor cells are filled through. The cell attachment areas in the PDMS microchannel have been recorded dynamically with 10 min interval continuously for 2 hours. The result show that 2 hour is not enough for the permanent cell attachment of HepG2 in this fractal PDMS microchannel.