Title :
On-chip manipulating and impedance spectroscopy sensing of single mouse embryo
Author :
Zhuoqi Li ; Xinwu Xie ; Weixing Chen ; Weiran Liu ; Ran Liu
Author_Institution :
Dept. of Biomed. Eng., Tsinghua Univ., Beijing, China
Abstract :
A microfluidic device for single mouse embryo manipulation was fabricated and the impedance spectroscopy of the positioned embryo was detected by the electrodes in the microchannel. Experiment results showed that the impedance of embryo varies when it is at different stage. Also, healthy embryos and development-blocked embryos showed different impedance spectroscopy. Compared with the traditional means of development evaluation in clinic, sensing of impedance is a totally new method for embryo development with the benefit of label free, quantifiable, real-time monitoring and had great potential to be used in development status prediction in clinic.
Keywords :
bioMEMS; electrochemical impedance spectroscopy; lab-on-a-chip; microchannel flow; microelectrodes; development status prediction; development-blocked embryos; electrodes; healthy embryos; impedance spectroscopy; microchannel; microfluidic device; on-chip manipulation; single mouse embryo manipulation; Electrodes; Embryo; Impedance; Mice; Microfluidics; Sensors; Spectroscopy;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2014 9th IEEE International Conference on
Conference_Location :
Waikiki Beach, HI
DOI :
10.1109/NEMS.2014.6908795
