Title :
Pulsatile shear stress and high glucose concentrations induced reactive oxigen species production in endothelial cells
Author :
Yu, J.Q. ; Chin, L.K. ; Fu, Y. ; Yu, T. ; Luo, K.Q. ; Liu, A.Q.
Author_Institution :
Sch. of Electr. & Electron. Eng., Nanyang Technol. Univ., Singapore, Singapore
Abstract :
A hemodynamic Lab-on-a-chip system was developed in this study. This system has two unique features: (1) it consists of a microfluidic network with an array of endothelial cell seeding sites for testing them under multiple conditions, and (2) the flow rate and the frequency of the culture medium in the microchannel are controlled by a pulsation free pump to mimic the flow profile of the blood in the blood vessel under different physiological conditions. The results demonstrate the advantage of utilizing this system over the conventional non-pulsatile system in the future shear stress related studies.
Keywords :
bioMEMS; biochemistry; blood vessels; cellular biophysics; haemodynamics; lab-on-a-chip; microfluidics; organic compounds; pulsatile flow; stress effects; blood flow profile mimic; blood vessel; endothelial cell seeding site array; endothelial cells; glucose concentration induced ROS production; hemodynamic lab on a chip system; microchannel culture medium flow frequency; microchannel culture medium flow rate; microfluidic network; pulsatile shear stress induced ROS production; pulsation free pump; reactive oxygen species production; shear stress related studies; Biomedical monitoring; Cells (biology); Chemicals; Fluorescence; Stress; Sugar; Hemodynamic; Lab-on-a-chip; endothelial cell; reactive oxygen species;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International
Conference_Location :
Beijing
Print_ISBN :
978-1-4577-0157-3
DOI :
10.1109/TRANSDUCERS.2011.5969310
