Pulsatile shear stress and high glucose concentrations induced reactive oxigen species production in endothelial cells

Author

Yu, J.Q. ; Chin, L.K. ; Fu, Y. ; Yu, T. ; Luo, K.Q. ; Liu, A.Q.

Author_Institution

Sch. of Electr. & Electron. Eng., Nanyang Technol. Univ., Singapore, Singapore

fYear

2011

fDate

5-9 June 2011

Firstpage

767

Lastpage

770

Abstract

A hemodynamic Lab-on-a-chip system was developed in this study. This system has two unique features: (1) it consists of a microfluidic network with an array of endothelial cell seeding sites for testing them under multiple conditions, and (2) the flow rate and the frequency of the culture medium in the microchannel are controlled by a pulsation free pump to mimic the flow profile of the blood in the blood vessel under different physiological conditions. The results demonstrate the advantage of utilizing this system over the conventional non-pulsatile system in the future shear stress related studies.

Keywords

bioMEMS; biochemistry; blood vessels; cellular biophysics; haemodynamics; lab-on-a-chip; microfluidics; organic compounds; pulsatile flow; stress effects; blood flow profile mimic; blood vessel; endothelial cell seeding site array; endothelial cells; glucose concentration induced ROS production; hemodynamic lab on a chip system; microchannel culture medium flow frequency; microchannel culture medium flow rate; microfluidic network; pulsatile shear stress induced ROS production; pulsation free pump; reactive oxygen species production; shear stress related studies; Biomedical monitoring; Cells (biology); Chemicals; Fluorescence; Stress; Sugar; Hemodynamic; Lab-on-a-chip; endothelial cell; reactive oxygen species;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International

Conference_Location

Beijing

ISSN

Pending

Print_ISBN

978-1-4577-0157-3

Type

conf

DOI

10.1109/TRANSDUCERS.2011.5969310

Filename

5969310