3D lobule-mimetic chip via positive Dielectrophoresis force with sinusoidal spacing poly (ethylene glycol)-diacrylate microwalls

In vitro culture and patterning of living cells to form a ordered 3D tissue is a rapidly developing technique with its applications in drug testing, co-culture studies, generating in vitro models of disease and other fields of biotechnology. This paper reports a 3D lobule-mimetic chip where in a three-dimensional lobule is constructed by using positive DEP (Dielectrophoresis) force. Positive DEP force was utilized to attract, adhere and pattern the cells on the surface of poly (ethylene glycol)-diacrylate (PEG-DA). PEG-DA-based check valve and valveless holes were designed to control the distribution of cells. A 3D lobule-mimetic chip is observed depend on this geometry microstructure and the novel cell manipulation method.