Development of a microfluidic device with microcantilever sensor array for probing single cancer cells mechanics

Author

Yin, T.I. ; Zhao, Y. ; Lin, C.F. ; Tsai, H.H. ; Juang, Y.Z. ; Urban, G.A.

Author_Institution

IMTEK, Univ. of Freiburg, Freiburg, Germany

fYear

2011

fDate

5-9 June 2011

Firstpage

1216

Lastpage

1219

Abstract

A new design of microfluidic device with integrated microcantilever sensor array is proposed in this work for probing single cancer cells mechanics. Array of piezoresistive microcantilever with interdigitated microelectrode is used as a smart substrate for trapping cells by the mechanism of negative dielectrophoretic (nDEP) and for measuring the cellular contractile force induced by the trapped cells. The cantilever sensor design is optimized based on a mechanics model for higher cellular force sensitivity. Single cancer cells trapping and cellular contractile force measurement has been demonstrated under the normal cell culture medium and extracellular matrix coating on cantilever surface. By the proposed microfluidic device, a new platform for cell mechanics-based cancer research is promising.

Keywords

bioMEMS; biomechanics; cancer; cantilevers; microelectrodes; microfluidics; microsensors; piezoresistive devices; cantilever sensor design; cellular contractile force; interdigitated microelectrode; microcantilever sensor array; microfluidic device; negative dielectrophoretic; piezoresistive microcantilever; single cancer cells mechanics; Arrays; Cancer; Charge carrier processes; Force; Force measurement; Microfluidics; Stress; Cellular contractile force; Microfluidic device; Piezoresistive cantilever; nDEP cell trap;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International

Conference_Location

Beijing

ISSN

Pending

Print_ISBN

978-1-4577-0157-3

Type

conf

DOI

10.1109/TRANSDUCERS.2011.5969377

Filename

5969377