Title :
Longitudinal evaluation of the mechanical properties of biomineralized osteoblasts
Author :
Meng, Y. ; Ba, X. ; Ge, S. ; Pernodet, N. ; Rafailovich, M. ; Qin, Y.X.
Author_Institution :
State Univ. of New York, Stony Brook
Abstract :
Understanding how biomineralization occurs in the extracellular matrix (ECM) of bone cells is crucial to the development of a successfully engineered bone tissue scaffold, and to date there has not been a well-established method for the quantitative examination of bone mineralization in situ. We investigated the mechanical properties of MC3T3-E1 osteoblast-like cells in vitro using shear modulation force microscopy (SMFM). The elastic modulus of the mineralizing cells increased at time points corresponding to mineral production, whereas that of the non-mineralizing cells did not vary significantly over time.
Keywords :
Young´s modulus; atomic force microscopy; biomechanics; biomedical materials; biomineralisation; bone; cellular biophysics; orthopaedics; tissue engineering; MC3T3-E1 osteoblast-like cells; biomineralized osteoblasts; elastic modulus; engineered bone tissue scaffold; extracellular bone cell matrix; in situ bone mineralization; in vitro analysis; longitudinal evaluation; mechanical properties; shear modulation force microscopy; Atomic force microscopy; Atomic measurements; Biological materials; Biomedical engineering; Biomedical measurements; Bones; Force measurement; Mechanical factors; Mineralization; Thermal force;
Conference_Titel :
Bioengineering Conference, 2007. NEBC '07. IEEE 33rd Annual Northeast
Conference_Location :
Long Island, NY
Print_ISBN :
978-1-4244-1033-0
Electronic_ISBN :
978-1-4244-1033-0
DOI :
10.1109/NEBC.2007.4413257
