High-performance protein preconcentrator using microchannel-integrated nafion strip

Author

Yang, H. ; Shen, M. ; Sivagnanam, V. ; Gijs, M.A.M.

Author_Institution

Lab. of Microsyst., Ecole Polytech. Fed. de Lausanne, Lausanne, Switzerland

fYear

2011

fDate

5-9 June 2011

Firstpage

238

Lastpage

241

Abstract

A microfluidic device for protein preconcentration based on the electrokinetic trapping principle is proposed. It comprises a Nafion strip which is simply cut from a commercially available membrane and integrated into a polydimethylsiloxane (PDMS) microfluidic structure using a guiding channel. By applying low voltage (<;15 V), the device exhibits a preconcentration factor of >;10³ of fluorescently labeled bovine serum albumin (BSA) within a few minutes. Moreover, a numerical study of the preconcentration effect is presented to microscopically understand the phenomenon in our type of device.

Keywords

electrokinetic effects; microchannel flow; proteins; bovine serum albumin; electrokinetic trapping principle; high-performance protein preconcentrator; microchannel-integrated nafion strip; microfluidic device; polydimethylsiloxane microfluidic structure; Biomembranes; Electrokinetics; Fluorescence; Microfluidics; Nanobioscience; Proteins; Strips; Nafion; Protein preconcentrator; electrokinetic trapping; microfluidics;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International

Conference_Location

Beijing

ISSN

Pending

Print_ISBN

978-1-4577-0157-3

Type

conf

DOI

10.1109/TRANSDUCERS.2011.5969416

Filename

5969416