• DocumentCode
    2533891
  • Title

    A platform for in situ real-time measurement of protein induced conformational changes of DNA

  • Author

    Spuhler, Philipp S. ; Knezevic, Jelena ; Yalcin, Ayca ; Dröge, Peter ; Rant, Ulrich ; Ünlü, M. Selim

  • Author_Institution
    Biomed. Eng., Boston Univ., Boston, MA, USA
  • fYear
    2009
  • fDate
    4-8 Oct. 2009
  • Firstpage
    461
  • Lastpage
    462
  • Abstract
    A platform for in situ and real-time measurement of protein induced conformational changes in dsDNA is presented. We demonstrate protein induced bending upon sequence specific binding of integration host factor (IHF) to dsDNA probes.
  • Keywords
    DNA; bioMEMS; biosensors; lab-on-a-chip; molecular biophysics; molecular configurations; proteins; bending; dsDNA; dsDNA probes; in situ real-time measurement; integration host factor; protein induced-conformational changes; sequence specific binding; Biomedical measurements; DNA; Electric variables measurement; Electrodes; Fluorescence; Gold; Probes; Proteins; Sequences; Throughput;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    LEOS Annual Meeting Conference Proceedings, 2009. LEOS '09. IEEE
  • Conference_Location
    Belek-Antalya
  • ISSN
    1092-8081
  • Print_ISBN
    978-1-4244-3680-4
  • Electronic_ISBN
    1092-8081
  • Type

    conf

  • DOI
    10.1109/LEOS.2009.5343182
  • Filename
    5343182